MOTS-c

Choosing a supplier for MOTS-c research comes down to reproducibility: the same sequence, the same purity profile and the same handling documentation on every order. MOTS-c is supplied by Protide Health Lab as a lyophilised powder with a third-party high-performance liquid chromatography and mass spectrometry (HPLC-MS) purity report, and it is intended strictly for laboratory research use.

MOTS-c is a 16-amino-acid peptide encoded within the 12S ribosomal RNA region of mitochondrial DNA. It belongs to the mitochondrial-derived peptide family and, unusually for a peptide, is said to translocate to the nucleus under metabolic stress, where it is implicated in retrograde mitochondrial-to-nuclear signalling. The freeze-dried presentation keeps the peptide in a form that tolerates shipping at ambient temperature while remaining suitable for long-term storage at reduced temperature once it reaches the laboratory.

MOTS-c Key Takeaways

• Compound class: mitochondrial-derived hexadecapeptide (16 amino acids, MT-RNR1 encoded).
• Format: lyophilised research powder in a sealed glass vial.
• Vial sizes: 10mg, 20mg, 40mg.
• Purity: 99% (HPLC-MS verified, third-party tested).
• Price range: $65 to $195 per vial.
• Fulfilment: domestic US shipping with a batch certificate of analysis.
• Status: for laboratory research use only; not for human or veterinary use.

Table of Contents

1. What Is MOTS-c?
2. MOTS-c Specifications
3. Research Overview
4. Reported Mechanism of Action
5. Purity, Testing and Certificates of Analysis
6. Handling, Storage and Reconstitution
7. Available Sizes and Pricing
8. How to Order MOTS-c Online
9. Frequently Asked Questions
10. Related Research Peptides
11. References and Further Reading

What Is MOTS-c?

As a mitochondrial-derived hexadecapeptide (16 amino acids, MT-RNR1 encoded), MOTS-c is offered by Protide Health Lab as a research reagent rather than as a therapeutic product. Analytical release testing covers sequence identity by mass spectrometry and purity by reverse-phase HPLC, and the outcome is documented in the certificate of analysis that accompanies the vial.

Also known as: MOTS-c, MOTS-c peptide, mitochondrial open reading frame of the 12S rRNA-c, MRWQEMGYIFYPRKLR.

Because the material is sold for laboratory investigation, Protide Health Lab does not make any claim about how it should be used, and every vial is labelled with the research-use-only designation. What can be verified before purchase is the analytical record: identity, purity, batch number and the testing laboratory.

MOTS-c Specifications

Property	Details
Product Contents	MOTS-c Lyophilized Powder (99% purity) in 3mL vial.
Available Sizes	10mg, 20mg, 40mg
Peptide Type	Mitochondrial-derived hexadecapeptide (16 amino acids, MT-RNR1 encoded)
Purity	99% (HPLC-MS verified, third-party tested)
Molecular Formula	C 101 H 152 N 28 O 22 S 2
Molecular Weight	2,174.64 g/mol
Sequence	Met-Arg-Trp-Gln-Glu-Met-Gly-Tyr-Ile-Phe-Tyr-Pro-Arg-Lys-Leu-Arg
CAS Number	1627580-64-6
CID	146675088
Synonyms	MOTSc, MOTS c, Mitochondrial Open Reading Frame of the 12S rRNA-c
Appearance	White to off-white lyophilized powder
Storage	Store at ≤6°C, sealed, away from heat, light, and moisture.
Solubility	Soluble in water

The table above summarises the identity and handling parameters of the material as supplied. Sequence, molecular weight and any database identifiers are given so that the vial can be reconciled with an existing internal specification or with a literature reference.

Research Overview: AMPK activation and energy sensing

The research record for MOTS-c frames it as a mitochondrial-derived hexadecapeptide (16 amino acids, MT-RNR1 encoded). Interest is concentrated in the following domains, each of which is presented here as a summary of published laboratory investigation only. If you are looking for buy mots c online, compare the batch documentation rather than the headline purity figure: sequence identity, purity method and testing laboratory matter more than a single percentage.

AMPK activation and energy sensing

Research interest in AMPK activation and energy sensing centres on how MOTS-c behaves at the level of dose-dependent changes in cellular readouts. Because these endpoints are measured in controlled culture systems, results are usually reported against a vehicle control and a reference compound, which allows the contribution of the peptide itself to be separated from assay variability. This is one of the clearest reasons to insist on batch-level analytical documentation before designing an experiment.

Mitochondrial retrograde signalling

Within Mitochondrial retrograde signalling, investigators use MOTS-c to probe receptor occupancy and downstream second-messenger activity. Reported findings in this area are most useful when the preparation is fully characterised, since an unquantified purity difference between batches can easily be mistaken for a biological effect. Reproducibility in this line of work depends heavily on the consistency of the starting material across repeats.

Glucose uptake and insulin sensitivity

Studies grouped under Glucose uptake and insulin sensitivity generally treat MOTS-c as a tool for examining gene-expression shifts and protein-level endpoints. The literature in this area is not uniform: study designs differ in concentration range, exposure time and model system, so comparisons across papers should be made with attention to those parameters. For a laboratory working in this field, the practical requirement is a peptide whose sequence identity and purity have been independently confirmed.

Lipid oxidation and metabolic flexibility

In Lipid oxidation and metabolic flexibility, published work typically examines MOTS-c from the perspective of enzyme kinetics and substrate handling. Endpoints here are typically quantitative – fluorescence, luminescence, chromatographic signal or imaging counts – which makes reproducible material quality a practical requirement rather than a formality. This is one of the clearest reasons to insist on batch-level analytical documentation before designing an experiment.

Nrf2 and antioxidant gene expression

Research interest in Nrf2 and antioxidant gene expression centres on how MOTS-c behaves at the level of cell-migration, proliferation and viability measurements. Because these endpoints are measured in controlled culture systems, results are usually reported against a vehicle control and a reference compound, which allows the contribution of the peptide itself to be separated from assay variability. Reproducibility in this line of work depends heavily on the consistency of the starting material across repeats.

Ageing and metabolic-stress models

Within Ageing and metabolic-stress models, investigators use MOTS-c to probe signalling cross-talk between converging pathways. Reported findings in this area are most useful when the preparation is fully characterised, since an unquantified purity difference between batches can easily be mistaken for a biological effect. For a laboratory working in this field, the practical requirement is a peptide whose sequence identity and purity have been independently confirmed.

Reported Mechanism of Action

MOTS-c is reported to activate AMP-activated protein kinase (AMPK) by inhibiting the folate-cycle enzyme and thereby raising intracellular AICAR, a well-known AMPK activator. Downstream effects studied in the literature include increased glucose uptake, altered lipid oxidation and changes in the expression of antioxidant-response genes such as Nrf2 targets. Its nuclear translocation links it to transcriptional regulation of metabolic and stress-response programmes.

As with most research compounds, the mechanism described above is assembled from multiple published studies that used different concentrations, exposure times and model systems. Readers should treat it as a map of reported signalling behaviour for this mitochondrial-derived hexadecapeptide (16 amino acids, MT-RNR1 encoded) rather than as a definitive account of a single pathway.

Purity, Testing and Certificates of Analysis

The difference between a usable research reagent and a wasted experiment is often analytical – the presence of truncated sequences, deletion products or residual solvents can shift outcomes in ways that are hard to attribute after the fact. Each batch is accompanied by a certificate of analysis that reports the analytical method, the observed purity, the identity confirmation and the testing laboratory, so the data can be filed with your study records.

Independent analysis of this material reports 99% (HPLC-MS verified, third-party tested). The certificate of analysis records the method used, the chromatographic profile, the mass-spectrometric identity confirmation and the final purity value, and it is issued against the specific batch supplied. That distinction matters: an identity confirmation from mass spectrometry establishes that the principal peak corresponds to the expected molecular mass, while the chromatographic purity figure describes how much of the total peak area that principal peak represents. A third element of quality control, which is not visible on a purity number, is the water and salt content of the lyophilised powder – residual moisture and counter-ion content both change the effective concentration of the peptide when the vial is dissolved. If your protocol requires a specific acceptance threshold, verify it against the certificate of analysis for the batch you receive before beginning work, and file that document with your study records so the material can be traced later.

Handling, Storage and Reconstitution

Storing the lyophilised powder

Before reconstitution, keep the vial frozen at -20 degrees Celsius, shielded from light, with the stopper in place and desiccant nearby if the laboratory is humid. A reconstituted solution is far less stable than the dry powder, so keep it refrigerated at 2 to 8 degrees Celsius and treat the working window as short.

Reconstitution

Most peptides in this catalogue dissolve readily in bacteriostatic water or in a 0.9% benzyl alcohol solution; a preserved diluent is often preferred when a multi-use vial will be drawn from repeatedly.

To calculate a working concentration, divide the mass of peptide in the vial by the volume of diluent added. For example, a 10mg vial dissolved in 2 mL of diluent gives approximately 5 mg/mL per mL. A convenient shorthand is that a 10mg vial dissolved in 2mL of diluent gives a 5mg/mL stock, while the same vial dissolved in 5mL gives 2mg/mL. Add the diluent slowly down the wall of the vial rather than directing the stream onto the powder, swirl gently instead of shaking, and never vortex a peptide solution: mechanical shear and foaming both encourage aggregation.

Laboratory handling practice

Prepare and handle MOTS-c in an appropriate laboratory environment using clean technique. Work with gloves and eye protection, use dedicated glassware or single-use labware, and label every aliquot with the compound name, concentration, batch number and preparation date. When drawing from a multi-use vial, swab the stopper before and after use and keep the needle small to reduce coreing. Avoid repeated freeze-thaw cycles, because each thaw exposes the peptide to a fresh round of hydrolysis and aggregation; prepare single-use aliquots instead. Dispose of all peptide waste in accordance with your institution’s chemical-waste policy, and record the batch number in your laboratory notebook so that any analytical outlier can be traced back to a specific vial.

Available Sizes and Pricing

Vial size	Price	Availability
10mg	$65	In stock
20mg	$110	In stock
40mg	$195	In stock

Choosing a size is largely a question of how many separate preparations your protocol requires. Consolidating into one larger vial reduces handling steps and the associated risk of variability, while small vials keep unused material in the more stable dry state for longer.

How to Order MOTS-c Online

Domestic orders are processed promptly, packed discretely and shipped with tracking, with the certificate of analysis included for your records. Browse the full Peptides category listing, or compare other compounds in the same family: 5-Amino 1-MQ, NAD+ / MOTS-C / 5-Amino-1MQ “Mitoprime” Blend, NAD+.

Protide Health Lab supplies MOTS-c from domestic inventory with same-day dispatch for orders received before the daily cut-off. Each order includes the batch-matched certificate of analysis, and shipment tracking is provided so that delivery can be confirmed. Keep the vial closed until it reaches the laboratory, then store it as described in the handling section above.

Frequently Asked Questions

What is MOTS-c?

MOTS-c is a 16-amino-acid peptide encoded within the 12S ribosomal RNA region of mitochondrial DNA It is supplied as a research reagent for in-vitro laboratory investigation, not as a therapeutic product..

What purity is MOTS-c supplied at?

The material is released against independent analytical testing, with a reported purity of 99% (HPLC-MS verified, third-party tested). The certificate of analysis for your specific batch records the method used, the chromatographic profile and the mass-spectrometric identity confirmation, and it ships with the order.

What vial sizes of MOTS-c are available?

MOTS-c is offered in 3 configurations: 10mg, 20mg, 40mg. The larger sizes suit repeat runs that benefit from a single stock preparation, while smaller vials keep unused material in the stable lyophilised state for longer.

How much does MOTS-c cost?

Pricing runs from $65 to $195 per vial depending on the size selected, and the current price for each configuration is listed in the sizes and pricing table above. The certificate of analysis is included at no additional cost.

What is MOTS-c supplied as?

It is supplied as a lyophilised (freeze-dried) powder in a sealed glass vial. The dry format is the most stable way to ship and store a research peptide, and it keeps the material usable for longer than a pre-dissolved solution would.

How should MOTS-c be stored?

Keep the sealed vial frozen at -20 degrees Celsius, protected from light, until you are ready to use it. After reconstitution, store the solution at 2 to 8 degrees Celsius and use single-use aliquots to avoid repeated freeze-thaw cycling, which accelerates hydrolysis and aggregation.

What diluent should be used to reconstitute MOTS-c?

Bacteriostatic water is the usual choice; a preserved 0.9%% benzyl alcohol solution is a practical alternative when the same vial will be drawn from repeatedly. Add the diluent slowly down the vial wall, swirl gently and never vortex, because mechanical shear promotes aggregation. Divide the vial mass by the volume added to obtain your working concentration.

Is MOTS-c intended for human use?

No. This product is a research chemical supplied for in-vitro laboratory investigation only. It is not intended for human or veterinary use, and it is not a treatment, diagnostic or supplement.

How can I confirm the certificate of analysis for my batch?

The certificate of analysis is matched to the batch number printed on the vial and is included with the shipment. Compare the reported purity and identity data against the acceptance criteria in your protocol and file the document with your study records so the material remains traceable.

Related Research Peptides

Researchers who work with MOTS-c frequently also examine the compounds below, either as alternatives within the same signalling family or as complementary reagents in a combined study design.

• 5-Amino 1-MQ – small-molecule quinolinium-class NNMT inhibitor.
• NAD+ / MOTS-C / 5-Amino-1MQ “Mitoprime” Blend – three-component mitochondrial and NAD+ research blend.
• NAD+ – pyridine dinucleotide coenzyme (beta-NAD+).
• IGF-1 LR3 1mg – synthetic long-arginine analogue of insulin-like growth factor 1 (83 amino acids).

The complete research catalogue is available on the https://protidehealthlab.store/shop/ page. If you are unsure which vial size fits your protocol, order the smaller size first, confirm that the material meets your acceptance criteria against the certificate of analysis, and scale up once the preparation has performed as expected. Every listing shows the analytical documentation for the batch, so the material can be evaluated on evidence before it is ordered.

References and Further Reading

The links below point to publicly indexed literature and reference resources relevant to this compound class. They are provided so that the mechanistic summaries above can be traced to their sources. Access to full text depends on the subscription arrangements of your institution.

• Peer-reviewed literature indexed in PubMed (PMID 25738459)
• Peer-reviewed literature indexed in PubMed (PMID 29983246)
• PubMed – biomedical literature database maintained by the National Library of Medicine
• National Institutes of Health – public research and health information resources

Research Use Only Disclaimer

This product is sold strictly as a chemical reference material for in-vitro laboratory research and is not a drug, supplement, cosmetic or food. It must not be administered to humans or animals, must not be used for diagnostic or therapeutic purposes, and must be handled only by qualified personnel in an appropriate laboratory setting. Statements describing areas of scientific interest are summaries of published research literature and do not constitute claims of efficacy, safety or intended use. By purchasing, the buyer confirms that they are a qualified researcher and that the material will be used lawfully within an institutional or laboratory research context.